Article Snippet: A Rotor-Gene 6000 real-time rotary analyzer (Corbett Life Science, Australia) was used to amplify both target and internal control templates (1 cycle at 95☌ for 5 min and 40 amplification cycles at 95☌ for 30 sec, 55☌ for 30 sec and 72☌ for 45 sec.
Rotor gene 6000 price plus#
Together with K + and other cations, Factor MP stabilizes specifically bound primers, allowing efficient primer extension by HotStarTaq Plus DNA Polymerase. rotor gene 6000 real time rotary analyzer - by Bioz Stars, 2022-04 86 / 100 stars. Synthetic Factor MP, an innovative PCR additive, increases the local concentration of primers at the template. In contrast to current methods, the kits eliminate the need for optimization of the concentrations of primers, Mg 2+, and Taq DNA polymerase, even for difficult assays (e.g., assays in which the copy number of the target gene is much smaller than that for the reference gene).| NH 4 + ions prevent nonspecific primers from annealing to the template. Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing primer annealing time.|Rotor-Gene Multiplex RT-PCR Kits provide a simple procedure for quantitative, multiplex, real-time PCR. In addition, the unique buffer composition supports the melting of DNA, reducing denaturation and extension times. The amplification plots for the duplex reactions (colored) overlapped with those for control singleplex reactions (gray), demonstrating the reliability of the duplex analysis.| Q-Bond in Rotor-Gene Multiplex PCR Master Mix increases the affinity of DNA polymerase for short single-stranded DNA, reducing primer annealing time to a few seconds. Triplicate reactions were run on the Rotor-Gene 3000 using leukocyte RNA as template (10-fold dilutions from 100 ng to 10 pg).
SYBR Green- or Dye-Based One-Step qRT-PCR.Reverse Transcription & cDNA Synthesis for qPCR.Tagged Protein Expression, Purification, Detection.
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